Glycoside Hydrolase Family 92: α-mannosidase

α-1,2-mannosidase (e.g. EC 3.2.1.24) catalyzes the hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides (KEGG).

Here you can download all necessary files providing the results above:

Download of the alignment (Nexus format): click here

Download of the alignment (Fasta format): click here

Following primer were used (F - forward, R - reverse):

Amino acid motif

TGENPLW

TQGGSNA

Nucleotide sequence (5’-3’)

ACI GGI GAR AAY CCN YTN TGG

GC RTT ISW ICC ICC YTG NGT

Specificity

Ascomycetes

Primer

GH92_350F

GH92_420R

Protein characteristics are:

Size: ~xx kDa, (xx, xx)

pI: x.x (xx)

catalytic center: glutamic residue

Reaction: here

CAZy GH92 family: here

no fungal 3D structure available

Findings:

In our study we found 9 different expressed α-mannosidase genes in the organic horizon of the forest soil; all of them cluster to ascomycete references (see Fig. 2). One type was found in both treatments (type 1).

Fig. 2: The evolutionary history was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 2.49189798 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (2000 replicates) are shown next to the branches. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). There were a total of 73 positions in the final dataset. Phylogenetic analyses were conducted in MEGA4.

References