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|
Study
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Site / forest type
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Compartments
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RNA/DNA
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total seqs / samples
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outcome
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|
Luis et al. 2004
|
Germany, Beech-Oak
|
O, A, B
|
DNA
|
~250 (64 types) of 5 cores
|
method developed
|
|
Luis et al. 2005a
|
Germany, Beech-Oak
|
Org. horizon
|
RNA
|
~120 (14 types) of 5 cores
|
method developed
|
|
Luis et al. 2005b
|
Germany, Beech-Oak
|
O, A, B
|
DNA
|
~600 (167 types) of 13 cores
|
diversity effects horizontally and vertically, saprotrophs dominate the O, mycorrhizals the A&B horizon
|
|
Blackwood et al. 2007
|
USA, Oak/Sugar Maple/Basswood
|
O
|
DNA
|
~60 (21 types); qPCR & LH-PCR, copy no. 0.5-8 Mio/g; 3 sites, 3 reps, 3 treat- ments, 2 seasonal samplings
|
|
|
Hofmockel et al. 2007
|
USA, Oak/Sugar Maple/Basswood
|
O, A
|
DNA
|
qPCR & LH-PCR, copy no. 0.1-10 Mio/g; 3 sites, 3 reps, 3 treatments, 4 samplings
|
|
|
Kellner et al. 2008
|
Germany, Beech-Oak & Grassland
|
O, A, B & Grsld upper layer
|
DNA
|
bacterial Laccases; ~170 (143 types) of 6 cores
|
high diversity of bacterial lcc in forests recorded, function not clear
|
|
Kellner et al. 2009
|
Germany, Beech
|
Org. horizon
|
DNA&RNA
|
>400 (73 DNA, 42 RNA types) of 18 cores (3 reps, 6 samplings)
|
seasonal changes of lcc diversity and expression in beech forest
|
|
Hassett et al. 2009
|
USA, Michgan, Sugar Maple
|
O, A
|
DNA
|
qPCR & LH-PCR, copy no. from 0.5-50 Mio/g; 4 sites (total 12 control, 12 N-plots)
|
|
|
Lauber et al. 2009
|
USA, Oak/Sugar Maple/Basswood
|
O
|
DNA
|
>200; qPCR, copy no. 3-4 Mio/g; 3 sites 3 reps, 3 treatments
|
|
|
Artz et al. 2009
|
Australia, Eucalyptus forest
|
upper soil layer
|
DNA
|
~200 sequences, 95 OTU; 18 sites: 6 control, 6 2yr burning, 6 4yr burning
|
burning declines phenoloxidase activity, but increases basidiomycete laccase gene diversity and eveness
|
|
Kellner et al. 2010
|
USA, Michgan, Sugar Maple
|
O
|
RNA
|
few expressed types, 1 field site, 3 control, 3 N-plots
|
expressed laccases in control and in N-plots, besides many other enzymes
|
|
Theuerl et al. 2010
|
Germany, Spruce
|
O (Oe,Oa), A, B
|
DNA
|
>200 (92 types), 3 treatments (plots), 3-5 to pool/plot, 2 seasonal samplings
|
response to reduced N, also roof effects, no changes in enzymes and decomposition
|
|
Ausec et al. 2011
|
Slovenia, Fen & Bog soil
|
upper 30cm
|
DNA
|
bacterial Laccases, 27-55 seqs per soil core, 3 fen, 3 bog cores, 241 total seqs
|
1/3 lcc types probably from Acidobacteria; novel 2-domain laccases; high diversities
|
|
Christ et al. 2011
|
Germany, Beech & Spruce
|
B, rocks
|
DNA
|
86 types in bulk soil, 39 types on stones, 8 sites sampled
|
differences soil vs. stone, pH effects, weathering of stones unlikely
|
|
Edwards et al. 2011
|
USA, Michgan, Sugar Maple
|
O
|
RNA
|
4 sites (3 controls, 3 N-plots) in 2009, one site 2007; qPCR
|
downregulation of laccase under increased N deposition
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