#NEXUS

Begin DATA;
	Dimensions ntax=31 nchar=496;
	Format datatype=NUCLEOTIDE gap=-;
	Matrix
	[                                             1         11        21        31        41        51        61        71        81        91        101       111       121       131       141       151       161       171       181       191       201       211       221       231       241       251       261       271       281       291       301       311       321       331       341       351       361       371       381       391       401       411       421       431       441       451       461       471       481       491      ]
	[                                             |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |      ]
	'Morchella_crassipes_14J_famI1_(AJ715440)'    ACCACGGTCCACTGGCACGGGTTCTTTCAACAGCATACCAATTACGTTGACGGCCCTGCATTCGTTACCCAGT-------------------------------------------------------------------------------------------------------------------------------------------GCCCTATCGTGCCAGGAAGCAGCTTTCCTTATGATTTTCAGGCG---------------------------------------------------------------------------------------GTAGACCAG-------------------------------------------------------------GCAGTATGGATTTCGTATCACGCACAGTTTCTGCCATTGACCTCTCTTATCTTACAG----GGCACTTTCTGGTACCACAGCC
	'Morchella_crassipes_10J_famI1_(AM269476)'    ACCTCGGTCCACTGGCACGGGTTTTTCCAACAACATACCAACTACGTCGATGGCCCTGCATTCGTTACTCAGT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGGAAACAGCTTCCTCTACGAGTTCGAGGCG---------------------------------------------------------------------------------------GTCAACCAG-------------------------------------------------------------GCGGTAAGAGACTTTAGACATCGGACAGGTTCAGGCTCTACATTTTATCCTGCAG------GGGACCTTCTGGTACCACAGTC
	'Morchella_crassipes_30J_famI1_(AM269477)'    ACCTCGGTTCACTGGCACGGCTTCTTCCAACAACATACCAACTACGTCGATGGCCCTGCATTCGTTACTCAGT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGGAAACAGCTTCCTCTACGAGTTCGAGGCG---------------------------------------------------------------------------------------GTCAACCAG-------------------------------------------------------------GCGGTAAGAGACTTTAGACATCGGACAGGTTCAGGCTCTACATTTTATCCTGCAG------GGAACGTTCTGGTACCACAGCC
	'Morchella_elata_Can1_var1_famI1_(AM269478)'  ACCTCGGTTCACTGGCACGGGTTTTTTCAACAACACACCAACTACGTCGATGGCCCCGCATTCGTTACCCAGT-------------------------------------------------------------------------------------------------------------------------------------------GCCCTATAATTCCAGGAAACAGCTTTCTCTACGAGTTCGAGGCG---------------------------------------------------------------------------------------TTCAACCAG-------------------------------------------------------------GCGGTAAGAGACTCTAGATCCACAGACTCAGGCGCTTCATCTGTAGTATCCTGCAG-----GGCACCTTCTGGTACCACAGTC
	'Morchella_elata_Can1_var2_famI1_(AM269479)'  ACCACGGTTCACTGGCACGGGTTTTTCCAACAACATACCAACTACGTCGATGGCCCTGCATTCGTTACTCAGT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGGAAACAGCTTCCTCTACGAGTTCGAGGCG---------------------------------------------------------------------------------------GTCAACCAG-------------------------------------------------------------GCGGTAAGAGACTTTAGACATCGGACAGGTTCAGGCTCTACATTTTATCCTGCAG------GGCACTTTCTGGTACCACAGCC
	'Morchella_crassipes_30J_famI2_(AM269480)'    ACCTCGGTTCACTGGCACGGGTTCTTCCAACATGGTACAAACTACAACGATGGGCCTGCCTTCGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGAGAATTCTTACCAGTATGACTTCGAGGTT---------------------------------------------------------------------------------------CTAGACCAGGTCCGTGTCGCATCTTGAGTGGTTATATTCCTACATTCCTACAGATCATCCAATCAACTAGGCT----------------------------------------------------------GGCACTTTCTGGTACCACAGCC
	'Morchella_elata_Can1_famI2_(AM269481)'       ACCTCGGTTCACTGGCACGGGTTCTTCCAACATGGTACAAACTACAACGATGGGCCTGCCTTCGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGAGAATTCTTACCAGTATGACTTCGAGGTT---------------------------------------------------------------------------------------CTAGACCAGGTCCGTGTCGCATCTTGAGTGGTTATATTCCTACATTCCTACAGATCATCCAATCAACTAGGCT----------------------------------------------------------GGGACGTTCTGGTACCACAGTC
	'Morchella_gigas_7MIJ3_famI2_(AM269482)'      ACCACCGTTCATTGGCACGGGTTCTTCCAACATGGTACAAACTACAACGATGGGCCTGCCTTCGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCATTCCAGAGAATTCTTACCAGTATGACTTCGAGGTT---------------------------------------------------------------------------------------CTAGACCAGGTCCGTGTCGCATCTTGAGTGGTTATATTCCTACATTCCTACAGATCATCCAATCAACTAGGCT----------------------------------------------------------GGGACGTTCTGGTACCACAGTC
	'Morchella_crassipes_30J_famI3_(AM269483)'    ACCACTGTTCATTGGCACGGATTTTTCCAAAGGGGTACAAACTATCAAGATGGGGCGAGTATGATTACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCGTTCCGAATAGCTCTTACCAGTACGACTTCACGGCT---------------------------------------------------------------------------------------CTCAATCAGGTCCGTGTCGATTGTTGGTGACGACATTGCATACTGATGAAGTAACCTGTTAG--------GCG----------------------------------------------------------GGAACGTTCTGGTACCACAGCC
	'Morchella_elata_Can1_famI3_(AM269484)'       ACATCGGTCCACTGGCACGGGTTTTTCCAAAGGGGTACAAACTATCAAGATGGGGCGAGTATGATTACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATCGTTCCGAATAGCTCTTACCAGTACGACTTCACGGCT---------------------------------------------------------------------------------------CTCAATCAGGTCCGTGTCGATTGTTGGTGACGACATTGCATACTGATGAAGTAACCTGTTAG--------GCG----------------------------------------------------------GGAACGTTCTGGTACCACAGCC
	'Morchella_gigas_7MIJ3_famIu_(AM269485)'      ACATCTGTTCACTGGCATGGCTTTTTCCAACATACTACAAACTACGTTGATGGGCCCTCATTTATTACCCAGT-------------------------------------------------------------------------------------------------------------------------------------------GTCCGATCGTGCCAGACAATTACTTCAACTACAACTTCCAGGCT---------------------------------------------------------------------------------------CTAAACCAG-------------------------------------------------------------GCGGTAATACATTGATCGCTACGAAGTATCCGTCCACCTATTGACTCGCCACGACATGCAGGGCACCTTCTGGTACCACAGCC
	'Morchella_crassipes_14J_famIu_(AM269486)'    ACCTCGGTCCATTGGCACGGGTTCTTTCAGAGTACAACCAACTGGGCAGATGGGCCAGCCTTTGTGACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCAATAATGCCCAACGACTCGTTCAGCTATGAGTTTGAAGTC---------------------------------------------------------------------------------------CCGAACCAA-------------------------------------------------------------GCC----------------------------------------------------------GGAACCTTCTGGTACCACAGCC
	'Morchella_gigas_7MIJ3_famIu_(AM269487)'      ACATCTGTTCACTGGCACGGGTTTTTCCAACACCATACGAATGCCTTTGACGGAACTGCTTTCGTGACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATTGTCCCTGGGAATTCTTTCCGCTATCAGTTCAATGTG---------------------------------------------------------------------------------------CAGAACCAG-------------------------------------------------------------GCT----------------------------------------------------------GGAACGTTCTGGTACCACAGCC
	'Verpa_conica_VE3J_famIu_(AJ715433)'          ACATCTGTTCATTGGCACGGGTTCTTTCAAAATCACACCAACTACGACGACGGTCCTGCTTTTGTCACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GTCCCATTATACCAAACGAAAGCTTTACGTATGACTTTTCGATC---------------------------------------------------------------------------------------CCGGACCAA-------------------------------------------------------------GCGGTATCTCCCCTTTTTCACGTCGAACGTGTCCGCGAGCTCACTGACTTGCTTATCCAG-GGGACCTTCTGGTACCACAGCC
	'Morchella_crassipes_10J_famII_(AM269488)'    ACCTCTGTCCATTGGCACGGCCTCTTCATGAATGGAACGAATTATATGGACGGACCCGTGGGAGTAACTCAGT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGCCAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACTTCAAATTATTTCTAGATCAATGCCTCGAGCCGAGATACTAACGAAAGCATCAATCACAG--------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGCACATTCTGGTACCACAGCC
	'Morchella_crassipes_30J_famII_(AM269489)'    ACAACTGTTCATTGGCACGGCCTCTTCCTGAATGGAACGAATTATATGGACGGACCCGTGGGAGTAACTCAGT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGCCAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACTTCAAATTATTTCTAGATCAATGCCTCGAGCCGAGATACTAACGAAAGCATCAATCACAG--------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGCACATTCTGGTACCACAGCC
	'Morchella_conica_22J1_famII_(AJ704782)'      ACCTCCGTTCACTGGCACGGGTTTTTCATGAATGGGACGAGTTACATGGATGGGCCCGTGGGAGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCGCGGTTGCGCCAGGACAGAGCATCGTATATGATTTC---GTTGTAGCTATTCACCTTCTCAACTTTTTTTCTTTCTCTTCAGATAAATGCTTGCTTCATGATGCTGATGAAACCACAAAACCGCAG---GTCGACCAG-------------------------------------------------------------CCA----------------------------------------------------------GGGACCTTCTGGTACCACAGCC
	'Morchella_elata_MC1SSI6_famII_(AJ715441)'    ACCTCGGTCCATTGGCACGGTCTCTTCATGAATGAAACAAACTACATGGATGGACCTGTAGGAGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCGCAGTTGCACCAGGACAGAGCATCGTGTATGATTTC---GTTGTAGGTATTCACCTTTCAATCTTTTTCTCTTGAATAAATGCTTCATGACACTGATGAAACCCACAAAACCTCAG-------------GTTAATCAG-------------------------------------------------------------CCA----------------------------------------------------------GGCACCTTCTGGTACCACAGCC
	'Morchella_elata_Can1_var1_famII_(AM269490)'  ACCTCGGTCCACTGGCACGGTCTCTTCATGAATGAAACAAACTACATGGATGGACCTGTAGGAGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCGCAGTTGCACCAGGACAGAGCATCGTGTATGATTTC---GTTGTAGGTATTCACCTTTCAATCTTTTTCTAATAAATGCCTCATGACACTGATGAAACCCACAAAACCTCAG-----------------GTTAATCAG-------------------------------------------------------------CCA----------------------------------------------------------GGCACCTTCTGGTACCACAGTC
	'Morchella_elata_Can1_var2_famII_(AM269491)'  ACATCGGTCCATTGGCACGGTCTCTTCATGAATGAAACAAACTACATGGATGGACCTGTAGGAGTGACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCGCAGTTGCACCAGGACAGAGCATCGTGTATGATTTC---GTCGTAGGTATTCACCTTTCAATCTTTTTCTAATAAATGCCTCATGACACTGATGAAACCCACAAAACCTCAG-----------------GTTAATCAG-------------------------------------------------------------CCA----------------------------------------------------------GGCACATTCTGGTACCACAGCC
	'Morchella_esculenta_A7_famII_(AJ715429)'     ACATCTGTCCATTGGCACGGCCTCTTCATGAATGGAACGAATTATATGGACGGACCCGTGGGAGTAACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCCGGACAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACTTCAAATTATTTCTAGACCAATACCCCGAGATACTAACGAAAGCGTCAATCACAG-------------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGCACTTTCTGGTACCACAGCC
	'Morchella_sp_MA4SSI73_famII_(AJ715442)'      ACCACGGTTCACTGGCACGGCCTCTTCATGAATGGGACGAATTATATGGATGGACCCGTGGGAGTAACCCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGACAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTTAACTTTAAATTATTTCTAGATCACTACCCCGAGATACTAACGAAAGCATCAATCACAG-------------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGCACCTTCTGGTACCACAGCC
	'Morchella_spongiola_5J5_famII_(AJ715427)'    ACCTCTGTTCACTGGCACGGCCTCTTCATGAATGGAACGAACTATATGGACGGACCCGTGGGAGTAACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGGCAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACATCAATTTATTTCTAAATCAATACCCCGAGATACTAACGAAAGCATCAATCACAG-------------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGAACCTTCTGGTACCACAGCC
	'Morchella_spongiola_7J1_famII_(AJ715428)'    ACCTCGGTCCATTGGCACGGCCTCTTCATGAATGGCACGAACTATATGGACGGACCCGTGGGAGTAACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGGCAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACATCAATTTATTTCTAAATCCATACCCCGAGATACTAACGAAAGCATCAATCACAG-------------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGCACCTTCTGGTACCACAGTC
	'Morchella_spongiola_Du3_famII_(AM269492)'    ACCTCGGTTCACTGGCACGGCCTCTTCATGAATGGAACGAACTATATGGACGGACCCGTGGGAGTAACTCAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCAGTTGCGCCGGGGCAGAGTATTGTATATGATTTT---GTTGTGAGATTACCTTGTCAACATCAATTTATTTCTAAATCAATACCCCGAGATACTAACGAAAGCATCAATCACAG-------------GTCGACCAA-------------------------------------------------------------CCA----------------------------------------------------------GGAACATTCTGGTACCACAGCC
	'Verpa_conica_VE3J_famII_(AJ715438)'          ACCACTGTCCACTGGCACGGCTTGTTCCAGAACGGGACGAATGCTATGGATGGGCCTATTGGTGCTACACAAT-------------------------------------------------------------------------------------------------------------------------------------------GCCCTGTGGCCCCGGGTCAGAGTATTGTGTATGATTTT---GTTGTCAGTCCTCTAGATCTGTCTAGAAACGAAAAATGCATAAAAATAATTACTATTTGGGAATCTCAAGATATTGATTTGGTGAAATAGGTTGATCAA-------------------------------------------------------------CCA----------------------------------------------------------GGAACCTTCTGGTACCACAGCC
	'Morchella_gigas_7MIJ3_famIII_(AM269493)'     ACATCTGTTCACTGGCACGGTCTGAGACAGCTCTGGACCACCGAGCAGGATGGCCTGAATGGAATCACTCAATGTAAGTCTGCCTCCACCCTCTGCTCTCCTCCTCCCCCCCCCACCACACTCCTTCCCCTTCTCCTCTTCCACCACACTCAAATATGCACAATACTTCAATGCTACAATAATACAACGGCTCAATATCTAACGCTCTCTAGGCCCAATTGCTCCCGCCGACAGCTTCACCTACGAATGGACAGCC------------------------------------------------------------------------------------------ACGCAA-------------------------------------------------------------TAC----------------------------------------------------------GGTACGTTCTGGTACCACAGCC
	'Verpa_conica_VE3J_famIII_(AM269494)'         ACATCTGTCCACTGGCACGGGATTAGACAGCTTTTGACCAATGAGGAGGATGGTGCTAATGGAGTCACTCAATGTGAGTTATTAGTCTAGCCCCAGATATTTAGGATTGTTGGCTAACTATCACAAG-------------------------------------------------------------------------------------GCCCGATTGCTCCTGGGCAACACCTTACCTATGAGTGGACCGCA------------------------------------------------------------------------------------------ACCCAA-------------------------------------------------------------TAC----------------------------------------------------------GGTACCTCTTGGTACCACTCTC
	'Morchella_conica_22J1_famIII_(AM269495)'     ACCTCGGTCCATTGGCACGGTCTGAGACAGCTCTGGACCACCGAGCAGGACGGTGTGAATGGTATCACTCAATGTAAGTTTCCCTTCCGTCTCACTCTCTCTTCTCTTTCCCACCACACTCCACACCCAAGTCTGCACAATAATACAATGGTTCAACATCTAACACTCCCCAG---------------------------------------GCCCAATTGCCCCCACCGATAGCTTCACCTACGAATGGACCGCT------------------------------------------------------------------------------------------ACTCAG-------------------------------------------------------------TAC----------------------------------------------------------GGTACCTCCTGGTATCACTCTC
	'Verpa_conica_VE3J_famIV_(AM269496)'          ACCTCGGTTCACTGGCACGGCCAATACCAGAATGGCACAAACTGGATGGACGGTACAACTGGGGTTACAAACT-------------------------------------------------------------------------------------------------------------------------------------------GCCCTATCCCCCCCGGAAAATCCTTCAGATATGAGTTCACTGTG---------------------------------------------------------------------------------------AGAGATCAA-------------------------------------------------------------CAC----------------------------------------------------------GGAACCTTCTGGTACCACAGCC
	'Gyromitra_esculenta_Gyr3_famIV_(AJ715432)'   ACCACTGTTCATTGGCACGGGCAGTATCAAAATGGCACCAACTTCATGGACGGCACAACAGGCATAACCAATT-------------------------------------------------------------------------------------------------------------------------------------------GCCCCATCCCTCCCGGCGCCTCCTATCCGTACGAGTTCACAGTC---------------------------------------------------------------------------------------CGGAAGCAG-------------------------------------------------------------TAC----------------------------------------------------------GGAACGTTCTGGTACCACAGCC

;
End;
begin Paup;
exclude 74-212 257-343 353-413 417-474; [introns are excluded]
exclude 1-21 475-496; [primer sites are excluded]
end;

Begin trees;  [Neighbor-joining distance analysis using K2P-distance matrix]
	Translate
		1 'Morchella_crassipes_14J_famI1_(AJ715440)',
		2 'Morchella_crassipes_10J_famI1_(AM269476)',
		3 'Morchella_crassipes_30J_famI1_(AM269477)',
		4 'Morchella_elata_Can1_var1_famI1_(AM269478)',
		5 'Morchella_elata_Can1_var2_famI1_(AM269479)',
		6 'Morchella_crassipes_30J_famI2_(AM269480)',
		7 'Morchella_elata_Can1_famI2_(AM269481)',
		8 'Morchella_gigas_7MIJ3_famI2_(AM269482)',
		9 'Morchella_crassipes_30J_famI3_(AM269483)',
		10 'Morchella_elata_Can1_famI3_(AM269484)',
		11 'Morchella_gigas_7MIJ3_famIu_(AM269485)',
		12 'Morchella_crassipes_14J_famIu_(AM269486)',
		13 'Morchella_gigas_7MIJ3_famIu_(AM269487)',
		14 'Verpa_conica_VE3J_famIu_(AJ715433)',
		15 'Morchella_crassipes_10J_famII_(AM269488)',
		16 'Morchella_crassipes_30J_famII_(AM269489)',
		17 'Morchella_conica_22J1_famII_(AJ704782)',
		18 'Morchella_elata_MC1SSI6_famII_(AJ715441)',
		19 'Morchella_elata_Can1_var1_famII_(AM269490)',
		20 'Morchella_elata_Can1_var2_famII_(AM269491)',
		21 'Morchella_esculenta_A7_famII_(AJ715429)',
		22 'Morchella_sp_MA4SSI73_famII_(AJ715442)',
		23 'Morchella_spongiola_5J5_famII_(AJ715427)',
		24 'Morchella_spongiola_7J1_famII_(AJ715428)',
		25 'Morchella_spongiola_Du3_famII_(AM269492)',
		26 'Verpa_conica_VE3J_famII_(AJ715438)',
		27 'Morchella_gigas_7MIJ3_famIII_(AM269493)',
		28 'Verpa_conica_VE3J_famIII_(AM269494)',
		29 'Morchella_conica_22J1_famIII_(AM269495)',
		30 'Verpa_conica_VE3J_famIV_(AM269496)',
		31 'Gyromitra_esculenta_Gyr3_famIV_(AJ715432)'
		;
tree PAUP_1 = [&U] (1,((((2,5),3),4),((((((6,7),8),((9,10),11)),13),(12,14)),((((((((15,16),((23,25),24)),21),22),26),17),((18,20),19)),(((27,29),28),(30,31))))));
End;